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| 半夏泻心汤调控Wnt/β-catenin通路改善2型糖尿病大鼠胰岛损伤的研究 |
| Study on the Effect of Banxia Xiexin Decoction in Regulating the Wnt/β-catenin Signaling Pathway to ameliorate pancreatic islet injury in rats with type 2 diabetes mellitus |
| 投稿时间：2026-09-30 修订日期：2026-10-13 |
| DOI： |
| 中文关键词: 半夏泻心汤 2型糖尿病 胰岛损伤 Wnt/β-catenin信号通路 细胞凋亡 |
| 英文关键词: Banxia Xiexin Decoction type 2 diabetes mellitus pancreatic islet injury Wnt/β-catenin signaling pathway apoptosis |
| 基金项目:2024年江西省中医药管理局科技计划项目 2024B1392 |
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| 中文摘要: |
| 目的 观察半夏泻心汤对2型糖尿病大鼠胰岛损伤的改善效应，并围绕Wnt/β-catenin级联探讨其分子机制。方法 以高脂饲料喂养叠加腹腔注射链脲佐菌素（STZ）的方案复制2型糖尿病大鼠，造模达标后归入正常对照、糖尿病模型、二甲双胍及半夏泻心汤低、中、高剂量6个组别；测定糖、脂代谢与胰岛功能相关参数，经HE染色评估胰岛形态学改变，并检测胰岛细胞凋亡程度及Wnt3a、β-catenin、CyclinD1、C-myc的蛋白水平。结果 半夏泻心汤可减轻糖脂代谢异常与胰岛组织损害，使胰岛细胞凋亡程度下降，并回调上述级联相关蛋白的表达。结论 半夏泻心汤对2型糖尿病大鼠胰岛具有保护作用，其机制可能与干预Wnt/β-catenin级联进而抑制胰岛细胞过度凋亡有关。 |
| 英文摘要: |
| Objective To observe how Banxia Xiexin Decoction alleviates pancreatic islet injury in type 2 diabetic rats and to probe the underlying molecular events along the Wnt/β-catenin cascade. Methods T2DM was reproduced in rats by feeding a high-fat diet together with an intraperitoneal injection of streptozotocin (STZ). Successfully modelled animals were assigned to six arms, namely a normal control arm, a diabetic model arm, a metformin arm, and three arms given low-, medium- and high-dose Banxia Xiexin Decoction. Glucose and lipid metabolic indices and islet function parameters were measured, islet morphology was assessed after hematoxylin and eosin (HE) staining, and both the extent of islet cell apoptosis and the protein levels of Wnt3a, β-catenin, CyclinD1 and C-myc were determined. Results Banxia Xiexin Decoction attenuated glucose and lipid metabolic disturbance and islet tissue damage, lowered the degree of islet cell apoptosis, and shifted the expression profile of the proteins belonging to the above cascade. Conclusion Banxia Xiexin Decoction protects pancreatic islets in T2DM rats, an effect that appears to involve interference with the Wnt/β-catenin cascade and consequent suppression of excessive islet cell apoptosis. |
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