| Qingjie Fuzheng Granules treat ulcerative colitis by regulating NLRP3 inflammasome-mediated pyroptosis |
| DOI： |
| En KeyWords: ulcerative colitis, Qingjie Fuzheng granules, NLRP3 inflammasome, pyroptosis |
| Fund Project:福建省自然科学基金(2022J01368) |
| Author:LIAO Zengmei |
| View Counts: |
| PDF Download Counts: |
| En Abstract: |
| Abstract Objective To observe the therapeutic effect of Qingjie Fuzheng Granules (QFG) on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) in mice through animal experiments, and to explore its mechanism of action. Methods Fifty male BALB/c mice were randomly divided into a blank group (Control group) of 10 mice and a model group of 40 mice. The Control group drank normal water, and the model group drank 3.5% DSS solution freely for 7 days and then were randomly divided into DSS group and DS group. The S+QFG group, DSS+NLRP3i group, and DSS+5-ASA group were given physiological saline, QFG, CY-09, and mesalazine solution by intragastric administration, once a day for 7 consecutive days. The body weight, fecal occult blood, and abdominal mass of the mice were measured every other day. Diarrhoea, disease activity index (DAI) was calculated; colon length, spleen weight and spleen weight index were measured after the intervention; colon tissue morphology and pathological scores were observed by hematoxylin-eosin staining (HE); IL-1β, I in serum were detected by Bio-Plex L-6, IL-17, and TNF-α contents; RT-qPCR detection of NLRP3, Caspase-1, IL-1β, IL-17, IL-18, and TNF-α mRNA expression in colon tissue; Western Blot detects the expression of Caspase-1 and GsderminD proteins in colon tissue. Results Compared with the Control group, the mice in the DSS group had reduced body weight, increased DAI score, shortened colon, increased spleen weight, increased spleen weight index (P<0.05), increased fecal occult blood score, destroyed colon crypt structure, inflammatory infiltration, increased histopathological score (P<0.05), increased levels of IL-1β, IL-6, IL-17, and TNF-α in serum (P<0.05), and increased levels of IL-1β, IL-6, IL-17, and TNF-α in colon tissue (P<0.05).The mRNA expression of IL-1β, IL-17, IL-18, TNF-α, NLRP3, and Caspase-1 increased (P<0.05), and Caspase-1 and GsderminD in colon tissue The expression increased (P<0.05); compared with the DSS group, each administration group had increased body weight, decreased DAI score, longer colon, decreased spleen weight, decreased spleen weight index (P<0.05), decreased fecal occult blood score, and destroyed the colon crypt structure. Inflammatory infiltration was improved, histopathological scores were reduced (P<0.05), serum levels of IL-1β, IL-6, IL-17, and TNF-α were reduced (P<0.05), and levels of IL-1β, IL-17, and I in colon tissue were reduced (P<0.05). The mRNA expression of L-18, TNF-α, NLRP3, and Caspase-1 decreased (P<0.05), and the protein expression of Caspase-1 and Gsdermin in colon tissue decreased (P<0.05). Conclusion QFG alleviates DSS-induced ulcerative colitis by regulating NLRP3 inflammasome and mediated pyroptosis. |
|
View Fulltext
Download PDF Viewer |
| Close |
|
|
|